5×SDS-PAGE Protein Loading Buffer (Odorless, Reducing)

5×SDS-PAGE Protein Loading Buffer(Odorless, Reducing) is the most commonly used sample buffer for Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) of denatured proteins. The product contains bromophenol blue, which can confirm the progress of electrophoresis.

In addition, the product contains odorless reducing agent instead of dithiothreitol (DTT) or mercaptoethanol, making the protein loading operation safer and healthier, and the water solubility is more stable.

Ship with wet ice; Store at -20°C; Valid for 12 months.

1. Dissolve 5× SDS-PAGE Protein Sampling Buffer in a water bath at room temperature or 37°C before use, store at room temperature immediately after dissolution in a water bath, avoid prolonged placement in a water bath, and store at -20°C after use.

2. Add the appropriate volume according to the ratio of protein sample: 5× SDS-PAGE Protein Sampling Buffer = 4:1. Note: This product can also be used directly for lysis of cell or tissue samples after dilution to 1×.

3. Heat at 95°C in a water or metal bath for 10 min to fully denature the proteins.

4. Allow the tube to reach room temperature and thoroughly mix before use;

5. The samples can be loaded directly after reaching to room temperature;

6. Electrophoresis can be stopped until the blue dye reaches the bottom of the gel.

1. This product may precipitate particles when stored at low temperatures, ensure that the product is fully rewarmed and dissolved evenly before use, and store at -20℃ as soon as possible after use.

2. Avoid direct contact between reagents and skin.

3. For your safety or health, please wear safety glasses, gloves, or protective clothing.

For Research Use Only!