In Situ Hybridization Fixation Solution (Plant)

In histopathology, the tissue must be promptly and effectively fixed before subsequent embedding and sectioning can be completed. Organization fixed role is to maintain the inherent morphology and structure of organization, cell, prevent bacteria corrosion and tissue autolysis, save the inherent material inside the cell, the cell protein coagulation, as far as possible to reduce or end of endogenous and exogenous enzyme reaction, keep the cell or tissue basically like living material, also can make the organization hardening, easy to repair the cut of the organization. For certain infectious specimens, can prevent the spread of disease; Preserve the gross specimen. Finally, fixation can enhance the effect of staining.

The main components of the in situ hybridization fixative (plant) of this product are formalin, glacial acetic acid and 70% ethanol, which is good for the fixation of plant tissues. This product is suitable for general root, stem, leaf, anther, ovary tissue section. DEPC treatment effectively inactivates RNase in the solution and is specifically used to immobilize samples required for subsequent nucleic acid in situ hybridization testing.

Transport at room temperature; Store in a cool and dry place, away from direct sunlight, valid for 24 months.

After the plant tissue is removed, it is rinsed with water and then placed in FAA fixing solution. Larger tissue needs to be cut into small pieces and fixed again. Generally, it is fixed in FAA fixative for more than 24 h. Small or young tissue can reduce the time appropriately according to the size of the material. If possible, the sample is immersed in the fixation solution and then the fixation can be promoted by vacuuming.

1. Use the product in a well-ventilated environment with proper protection to avoid inhalation.

2. To ensure that the fixation solution fully penetrates the tissue, additional vacuuming treatment can be added.

3. Please tighten the cap in time after use to prevent volatile active ingredients.

4. For your safety and health, please wear a lab coat and disposable gloves when operating.

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