Recombinant DNase I (RNase-free)

 

Product Name	Cat.No.	Spec.
Recombinant DNase I (RNase-free)	G3342-500U	500U

 

 

DNase I is an endonuclease that hydrolyzes phosphodiester linkages yielding oligonucleotides with a 5'-phosphate and a 3'-hydroxyl group. DNase I is calcium-dependent and can be activated by magnesium and divalent manganese ions. In the presence of magnesium ion, DNase I could randomly splice any site of double-stranded DNA. In the presence of divalent manganese ions, DNase I can splice DNA double strands at the same site, forming blunt ends, or sticky ends with 1 or 2 nucleotides.

 

 

1. Preparing RNA samples without DNA;

2. Destroy genomic DNA in RNA preparations prior to reverse transcription-PCR (RT-PCR).

3. In vitro T7, T3, SP6 and other RNA Polymerases catalyze the removal of DNA templates in the RNA post-transcription system;

4. Nick translationin DNA markers;

5. Generating libraries of random DNA fragments;

6. Apoptosis Tunel detection of partial splice of genomic DNA as positive control.

Features: specifically degrades DNA, but cannot degrade RNA.

Source: Recombinant expression of the pichia coli strain containing the Bovine Pancreatic DNase I gene.

Definition of enzyme activity: One unit is the amount of enzyme required for complete degradation of 1 µg pBR322 vector DNA in 10 minutes at 37℃.

Inactivation or inhibition: A 10-minute incubation at 75°C for complete inactivation of DNase I.

Purity: ≥95% by SDS-PAGE, RNase free; 10 U/uL.

 

 

Ship with wet ice; Store at -20℃ valid for 12 months.

 

 

Component	G3342-500U
Recombinant DNase I (RNase-free)	50 μL
10×DNase I Reaction Buffer	1 mL
25 mM EDTA	1 mL

 

 

Reference use (e. g. prepare RNA samples without DNA)

a. Add the following component into a sterile, nuclease-free tube on ice in the indicated order, mix gently and centrifuge briefly.

Component	Amount
RNA	1 μg
Recombinant DNase I (RNase-free)	1 U
10×DNase I Reaction Buffer	1 μL
RNase-free Water	to 10 μL

 

1. Incubate at 37℃ for 30 minutes.

2. Add 0.5 μL of 25 mM EDTA to stop the reaction;

3. DNase I was completely inactivated after incubation at 75℃ for 10minutes.

 

 

1. When using the product, the enzyme should be on ice and stored immediately after use at -20ºC.

1. For your safty and health,please wear safety glasses, gloves, or protective clothing.

 

For Research Use Only!