Ampicillin, Sodium Salt

This product is ampicillin sodium salt, the commonly used concentration of cell screening is 50-100μg/ml. Ampicillin, synonymous with Ampicillin, is a β-lactam antibiotic belonging to the penicillin family, which is also sensitive to β-lactamases. The latter can cleave the β-lactam ring of ampicillin. Ampicillin inhibits Gram-positive, negative and anaerobic bacteria in a broad spectrum. The bacteriostatic mechanism is to interfere with the activity of penicillin binding protein (PBP), which is involved in the final step of peptidoglycan synthesis and catalyze the formation of pentaglycine cross-links between alanine and lysine residues, which maintain cell wall integrity and maintain normal bacterial growth. In addition, Ampicillin is bactericidal only against growing Escherichia coli (Col) bacteria. Ampicillin is commonly used as a selective antibiotic in cell culture for the preparation of LB or LB plates containing Ampicillin and the sterilization of cultured cells. Ampicillin inhibits Gram-negative and Gram-positive bacteria with minimum inhibitory concentrations (MIC) of 0.03-3 μg/ml and 0.02-1.5 mg/ml, respectively. Autoclaved AGAR or media should be cooled to 45-50 ° C before ampicillin solution is added. Plates containing ampicillin can be stored at 2-8° C for up to 2 weeks. Ampicillin was stable for 3 days at 37℃.

Transport and storage at 2-8℃, valid for 36 months.

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