Multifunctional Imaging System

 

The SCG-W5000 is a comprehensive device that integrates chemiluminescence technology and gel imaging. it is equipped with a high-sensitivity cooled camera with 9 million pixels, enabling rapid, accurate, and high throughput detection and imaging of samples. it is widely used in the fields of life sciences, medicine, and environmental protection.

 

Chemiluminescence Imaging System:

1. Weak band signal, one-time imaging

2. Both strong and weak band signals can be achieved, and the desired results can be obtained

3. Source file can be saved, and the image can be adjusted at any time

4. High Efficient

 

Gel Imaging System:
Three modes and multiple parameters can be flexibly adjusted, resulting in clear and bright imaging
UV laser protective board, safe and convenient for gel cutting

 

 

Product Name		Imaging System
Cat.No.		SCG-W5000
Dimensions		400×371×700 mm
Camera	PixelResolution	9 million
	Resolution	3000×3000
	Pixel size	3.76×3.76μm
	Target size	1"(11.28×11.28 mm)
	Full Well Capacity	16.5ke-(HCG),50.5ke-(LCG)
	Sensitivity	877mv@1/30s
	ReadoutNoise	1.24e-(HCG),3.22e-(LCG)
	Dark Current	0.0003e-/s/pixel@-15℃
	Signal-to-Noise Ratio	42.2dB(HCG),47dB(LCG)
	ExposureTime	0.1ms~1h
	Binning Mode	1×1,2×2,3×3
	Grayscale	16-bit(65536 levels)
	Cooling	Relative to Ambient Temperature -40°c
	Camera Type	Black and White Camera
Lens	Aperture	F0.95-F16
	Focal Length	17mm
	Type	Motorized zoom lens
Light Source	Bright Field Light source	Downward-facing LED white light source, symmetrically distributed on both sides
	Ultraviolet light source	310nm LED array, providing uniform transmissive illumination.
Dark Box	Light isolation	Fully light-sealed, isolates environmental light.
	Door Control	The door control sensor can control the on/off of the bright field light source.
	Rotating disc	Switch the filter according to the current mode to match the applications of chemiluminescence and gelimaging.
	Field of View	Effective field of view for membraneimagingis136mmxl36mm (expandable to200mmx200mm if necessary) Effective field of view for proteingelimagingis136mmx 136mm (expandableto200mmx 200mmifnecessary) The effective field of view for nucleic acid gel imaging is 200mmx200mm
	Gel Cutting	After opening the door, the UV light source can be extracted and used with aUV protective board for cutting adhesive
Software Functions	Exposure Modes	High Quality: Image quality is the highest Standard: Balances image quality and exposure speed. High Sensitivity: Provides the fastest exposure speed.
	Auto Exposure	Intelligent exposure technology quickly determines the optimal exposure time. With the combination of time imaging and time accumulation functions, users can achieve the best image results with just one operation.
	Real-time imaging	Real-time presentation of the changes in sample signals during the exposure process. allowing for the observation of every detail of the capture. Overexposed areas will be indicated for samples with overexposure.
	Time imaging	After exposure is complete, each frame image within the exposure time can be generated Through precise retrospective adjustments, users can choose any frame image within that exposure time as the final output.
	Time Accumulation	For samples with insufficient exposure, users can choose to continue exposure after the initial exposure is completed, enabling the sample to receive additional exposure on top of the already exposed time.
IndustrialComputer		10.4 inches,1024x768,Windows operating system
ExternalInterfaces		USB 3.0×2
Operating Voltage		100V-240V
ProductPower		100W
Product Net Weight		25Kg

 

Notes
It is prohibited to touch or scratch the internal lenses of the dark box with hands or sharp objects
After placing the experimental samples, make sure to close the instrument's flip door to prevent external light from entering the dark box and affecting the experimental results
During imaging experiments, shaking the experimental table or instrument is prohibited to avoid impacting the image quality.
Pay attention to electrical safety. Pullingor moving the power cord during the experiment is prohibited
After the experiment is completed, clean the samples and any residues inside the dark box thoroughly

 

Cat. No.	SCG-W5000	SCG-W3000	SCG-W1000
Dimension	400×371×700 mm	400×371×700 mm	400×371×700 mm
Camera	Depth-cooled high sensitivity camera	Depth-cooled high sensitivity camera	High-sensitivity camera
Resolution	3000×3000,9 megapixels	3000×3000, 9 megapixels	3072×2048,6.3 megapixels
Pixel	3.76×3.76 μm	3.76×3.76 μm	2.4×2.4 μm
Shooting Area	Effective field of view for blotting film/protein gel: 136×136 mm (can be expanded to 200×200 mm if required); Effective field of view for nucleic acid gel: 200×200 mm.	Blotting Film 136×136 mm (expandable to 200×200 mm if required)	Nucleic Acid Gel / Protein Gel 140×140 mm
Cooling Temperature	Relative ambient temperature -40°C	Relative ambient temperature -40°C	-
Light Source	Bright-field Light Source: Downward-facing LED white light source, symmetrically distributed on both sides. UV Light Source: 310 nm LED array for uniform transmission illumination.	Downward-facing LED white light, symmetrically distributed on both sides	Bright-field Light Source: Downward-facing LED white light source, symmetrically distributed on both sides. UV Light Source: 310 nm LED array for uniform transmission illumination.
Industrial Computer	10.4 inches, 1024×768 Windows operating system	10.4 inches, 1024×768 Windows operating system	10.4 inches, 1024×768 Windows operating system
External Interface	2 USB3.0	2 USB3.0	2 USB3.0
Working Voltage	100 V-240 V	100 V-240 V	100 V-240 V
Product Power	100 W	100 W	100 W
Net Weight	25 kg	25 kg	30 kg
Real-Time Imaging	Yes	Yes	-
Time Imaging	Yes	Yes	-
Time Accumulation	Yes	Yes	-
Auto Exposure	Yes	Yes	Yes
Choice of 3 Imaging Modes	Yes	Yes	-
Protein Gel/Nucleic Acid Gel Imaging	Yes	-	Yes
Nucleic Acid Gel Cutting	Yes	-	Yes

 

Operating Procedures

Chemiluminescence Imaging Application

Power on

Plug in the power cord and turn on the power switch at the back of the instrument. The industrial computer will start up.

Sample Loading

Open the instrument door, take out the sample tray, place the prepared text sample on the tray, and then place the tray flat in the groove inside the instrument dark box. Closetheinstrumentdoor.

Launchinglmaging Software

After the industrial computer starts up, the application software will be automatically loaded. 0ncethe software is successfully launched, it will navigate to the main page.

 

The top-left section displays the company logo.
The bottom-left section is the status bar, showing the current camera and control board connection status, as well as detection of the inserted removable disk.

The top-right section displays the software version number.
The bottom-right section includes buttons for switching between simplified/traditional Chinese, switching to English, exporting the page, and closing the program.

Clicking on the central icon will enter the preview and capture page

Clicking on the Chemiluminescence will enter the preview and capture page

 

 

Preview and Capture Page

On the preview page, the user needs to input the location for storing the experimental results. The file name is optional and facilitates file retrieval for the user.

The user can choose between manual exposure and automatic exposure as the current experimental mode.

For manual exposure, the user needs to input the exposure time, while for automatic exposure, the algorithm calculates the optimal exposure time.

On the right side of the preview, the user can input a time value in microseconds (us). This time represents the exposure time for the bright field image. Clicking the preview switch initiates the preview, and the preview time can be adjusted as needed.

Clicking the capture button starts the exposure for capturing the image while clicking the return button takes the user back to the main page.

 

 

Shooting Process

1. Select automatic exposure and set the preview time.

Automatic exposure intelligent exposure technology can quickly determine the optimal exposure time.

2. Real-time making

Click on the capture button to start the exposure. A strip is displayed on the left, and a countdown of the exposure time is shown on the right. As the countdown progresses, real-time imaging of the sample signal changes is displayed on the left.

Real-time imaging Presents the changes in the sample signal during the exposure process in real-time, allowing users to grasp every detail of the capture. This breakthrough feature not only enhances shooting efficiency but also greatly improves user interaction experience.

During real-time imaging, areas in the strip that are overexposed will be displayed in red. lf it is determined that the strip meets the requirements, you can click on the stop button in the lower right corner to end the exposure early.

 

3. Temporallmaging, Time Accumulation

After the exposure is complete, it automatically enters the results page, where adjustments can be made to the captured results.

Temporal Imaging Through precise retrospective adjustment, users can select any frame within the exposure time as the final output result.

Time Accumulation Even after the exposure is complete, users can choose to continue the exposure, allowing the sample to receive additional exposure based on the already-exposed time. When clicking "Continue Exposure," there is a prompt for the minimum exposure time. The set time needs to be greater than this minimum time. lf the set time is shorter than this minimum time, the actual exposure time will be the minimum exposure time indicated by the prompt.

 

Image adjustments, result saving

After obtaining satisfactory images through automatic exposure, continued exposure, and temporal imaging adjustments can be made to the brightfield image, dark field image, and composite image on the results page.

Contrast Adjust the brightness and darkness of the bright field image

 

Exposure Adjust the lightness and darkness of the dark field image, Clicking on "auto-adapt" can recommend the most suitable value

Rotation Rotate the image

Invert Apply an inverted color effect to the image, turning black into white and white into black

Crop Clicking on the crop will generate a region on the image. This region can be resized, and after selecting the appropriate size clicking on crop again will crop out the desired area

Vertical flip Flips the image vertically

Horizontal flip Flips the image horizontally

Restore Restores the image to its original state

Im port Select the file automatically saved by the program to import it into the program for further operations

Image saving Save the image and also save the bright field image, dark field image, and composite image

Result saving Save the adjusted image as an encrypted file

If any adjustments are made to the image on the results page

 

 

Export Page

 

 

Storage location Select the location of the image files automatically saved by the system, and the contents of the files will be displayed in a list format the system

Open file Open the selected directory

Export Selecttheexport contentandformat to exportthe images

Delete Select the image file to be deleted,and it will be deleted

Open Select the corresponding file in the list to open and jump to the result page. You can operate on the image on the result page, or double-click the corresponding file to open it

Return Click "return" to return to the main page

 

 

Power on

Plug in the power cord, turn on the power switch at the back of the instrument, and the industrial computer will start up.

Sample Loading

Protein Gel Open the instrument door, take out the white sample tray, place the prepared sample to be tested on the tray, then place the tray flat in the groove inside the instrument dark box, and close the instrument door.

Nucleic Acid Gel Open the instrument door, place the sample on the ultraviolet transmissive glass of the ultraviolet light source module, with markings on the surface of the ultraviolet glass indicating the range for placing the sample.

Load Application software

After the industrial computer starts up, the application software is automatically loaded. Once the software starts up normally, it will navigate to the main page.

The upper left part displays the company;

The lower left part is the status bar, showing the connection status of the current camera and control board, as well as the detection of inserted removable disks;

The upper right part displays the software version number;

The lower right part includes buttons for switching between simplified and traditional Chinese, switching to English, accessing the export page, and closing the program;

Clicking on the icon in the middle and selecting, "Gel lmaging" will take you to the preview and capture page.

 

 

Preview and Capture Page

 

Preview and capture pages include status display information, camera settings, lens settings, mode settings, image saving, and cropping, etc.

The top bar consists of a light source status indicator, keyboard open button, minimize, window restore, exit gel imaging; file path input to save the location of the image, file name input to save the name of the image.

The middle window displays real-time captured images from the camera.

The bottom bar includes camera/control board/mobile disk connection status display information, image format selection for saving (tiff/jpeg/bmp), save image button, open image path, and image cropping button.

The right is for camera settings, mode settings, and lens settings, which are described as follows:

Video settings Double-click" Video Settings" to center the image

Contrast Adjusts brightness, range -100~100, default value is 0, increasing the value makes the image brighter, decreasing the value makes it darker

Exposure Sets the exposure time in milliseconds, default time is 350ms, increasing the time makes the image brighter

Gain Sets the gain value, default value is 500%, range 100%~5000%, increasing the gain makes the image brighter

Auto Exposure In Automatic exposure mode, the camera automatically adjusts exposure time and gain

Manual Exposure Manual exposure mode, manually set exposure time and gain

Negative Film Inverts blackand white colors

lllumination Turnson/off the illumination LED, used for capturing protein gel mode

 

UV Light Turns on/off the UV light source, can only be operated when the door is closed, used for capturing nucleic acid gel mode

Gel cutting, Turns on/off the gel cutting mode, and can only be operated when the door is open, when gel cutting is turned on, the UV light source will also automatically turn on, UV protective plate must be placed before opening

 

Adjustment Mode Adiuststhefocusand aperture range of the motorized lens

Coarse Adjustment Large adjustment step size, focusing adjustment step size of400, aperture adjustment step size of 200, when the image is completely blurred, coarse adjustment can quickly bring the image to a relatively clear state

Fine Adjustment Medium adjustment step size, focusing adjustment step size of 200, aperture adjustment step size of40,after coarse adjustment,fine adjustment can make the image clearer

Ultra-fine Adjustment Small adjustment step size, focusing adjustment step size of 15, aperture adjustment step size of 15 can bring the image to the clearest state

Focus Adjusts the focal length range (0-4500) through the "." and "+" buttons, the value below indicates the focus motor adjustment step range and real-time step

Aperture Adjusts the aperture range (0-2150) through the "." and "+" buttons, the value below indicates the aperture motor adjustment strange and real-time step

 

 

1. Select auto exposure, default contrast is 0, default exposure is 350, default gain is 500.

2. Nucleic Acid Gel: Turn on UV light, open the UV light source, then the bands can be seen in the window

Protein Gel: Turn on illumination, open the bright field light source, then the bands can be seen in the window.

 

 

3. Adjust the focus to make the bands as clear as possible

4. The default aperture step is 1700, which can be adjusted as needed. It is not recommended to adjust it too large or too small. It is recommended to adjust it in the range of 1500-1900. The aperture is mainly used to adjust the brightness of the bands, lf adjusting the aperture does not achieve the desired brightness of the bands, switch to manual exposure and set the exposure time, which can significantly change the brightness.

5. Enter the file path and filename, then click"Save Image" to save the current image.

 

 

If only certain bands are needed, click on "image Crop" to enter the image cropping page. Slide the screen from the upper left corner of the desired band to its lower right corner to create a red box outlining the band. Double. clicking on any position within the red box will enlarge the band to fill the entire window.

Horizontal and vertical flips can be used to flip the bands along the horizontal and vertical directions, and 'Restore" can return the bands to their original state before flipping.

Once the bands are adjusted to the desired state, enter the file path and filename.

Click "Save image" to save the current image. Click"Exit" to return to the preview and shooting page.

 

 

Gel cutting

1. Open the instrument door, and the UV light source will automatically turn off.

2. Pull out the drawer containing the UV light source and place the UV protective shield diagonally on the drawer and the instrument to block the UV light source from direct exposure to the eves.

 

Click on "Cutting Gel," the UV light source will turn on, and at this point, the bands of nucleic acid gel can be observed, and the gel cutting action can be carried out.