Nylon Membrane

Our premium-grade Positively Charged Nylon Membrane is a high-performance synthetic polyamide film engineered for superior nucleic acid and protein binding in molecular biology applications. With a pore size of 0.45 μm and pre-cut dimensions of 15×15 cm, this membrane delivers exceptional sensitivity and reproducibility for critical diagnostic and research workflows. Its robust structure and strong positive charge ensure high-capacity binding of DNA, RNA, and proteins, making it an indispensable tool for laboratories requiring precision and reliability.

High Binding Capacity: Optimized surface charge enables efficient immobilization of nucleic acids (DNA/RNA) and proteins, ideal for low-abundance target detection.

Pre-Activated & Ready to Use: Hydrophilic design eliminates time-consuming ethanol/methanol activation steps-directly applicable for blotting procedures.

Enhanced Durability: Superior mechanical strength resists tearing during handling, transfer, or hybridization, even under high-stringency conditions.

Broad Compatibility: Supports non-radioactive detection methods (chemiluminescence, fluorescence) but incompatible with anionic dyes.

Technical Specifications

Material: Synthetic polyamide (nylon)

Pore Size: 0.45 μm

Dimensions: 15 cm × 15 cm (single sheet per pack)

Surface Charge: Permanent positive charge

Storage: Stable at ambient temperature; store in a dry environment to prevent moisture absorption.
 

This nylon membrane is optimized for:

Southern & Northern Blotting: Achieve sharp bands and minimal background noise for DNA/RNA analysis.

Electrophoretic Mobility Shift Assay (EMSA): Study protein-DNA interactions with high specificity.

Dot/Slot Blotting: Rapid screening of nucleic acid samples without electrophoresis.

Hybridization Workflows: Compatible with radioactive and non-radioactive probes for flexible experimental design.

As an ISO 13485-certified manufacturer with 15+ years of expertise in membrane technology, we serve leading biotech firms, academic institutions, and diagnostic centers globally. Our products undergo rigorous quality control to meet ISO standards, ensuring batch-to-batch consistency. 

A renowned cancer research institute utilized our nylon membranes to identify novel miRNA biomarkers in glioblastoma samples. The membrane's low background interference enabled clear detection of low-concentration targets, accelerating their breakthrough discovery.