RPMI-1640 Medium, Pen-Strep

RPMI refers to Roswell Park Memorial Institute. RPMI-1640 medium was originally developed to culture human leukemic cells in suspnesion and as a monolayer. RPMI-1640 medium is unique from other media because it contains reducing glutathione and high concentrations of vitamins. Rpmi-1640 medium contains biotin, vitamin B12 and para-aminobenzoic acid, which are not found in Eagle's Minimum Essential Medium or Dulbecco's Modified Eagle Medium. In addition, inositol and choline are present in very high concentrationd. Servicebio RPMI-1640 media preparations are available in with glucose or no glucose formulations, as well as with or without L-glutamine and sodium pyruvate. Servicebio RPMI-1640 media include phenol red as a pH indicator. All available RPMI-1640 media products contain a sodium bicarbonate buffer with or without HEPES to maintain optimal culture pH.

RPMI-1640 medium has been found sutiable culture for a variety of mammalian cells, including Hela, Juukat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas. RPMI-1640 contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI-1640 uses a sodium bicarbonate buffer system (2.0 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Penicillin can interfere with the synthesis of bacterial cell walls and is especially effective against gram-positive bacteria. Streptomycin can bind to the 30S subunit of the bacterial ribosome and inhibit the synthesis of bacterial proteins. It is effective against both gram-negative and Gram-positive bacteria, but it is particularly effective against Gram-negative bacteria. The combination of penicillin and streptomycin can prevent most bacterial contamination.

All raw material components used for Servicebio cell culture media products are screened through using strict quality control testing. Servicebio cell culture media are manufactured at a cGMP-compliant facility using water for injection, filtered through final 0.1 μm sterile filtration and aseptic filled in a Hundred-stage FFU. The filter integrity tests are performed before and after filtration. Each lot of cell culture media are subjected to sterility test, pH, osmolality, endotoxin and cell culture test.

The major character of this RPMI-1640 medium is as follows:

• With 11.1 mM Glucose, 2.05 mM GlutaPlus, phenol red.
• With 100 KU/L penicillin G sodium salt, 100 mg/L streptomycin sulfate
• Without sodium pyruvate and HEPES.

The complete formulation is available.

Ship at room temperature; Store at 2-8℃, valid for 12 months.

1. This product has been filtered and sterilized. Attention should be paid to aseptic operation during use to avoid contamination;

2. Avoid freezing the medium, as ice formation can lead to precipitation of nutrients, which may not fully dissolve upon thawing, affecting cell culture performance. Additionally, when storing the medium at 2-8°C in a refrigerator, do not place it directly against the inner wall to prevent local chilling that could cause the medium to freeze.For the best results, do not freeze and thaw repeatedly.

For Research Use Only!

RPMI-1640 Formula