Product Information
|
Name |
Cat No. |
Size |
|
Leibovitz's L-15 |
G4570-500ML |
500 mL |
Product Description
Leibovitz's L-15 Medium supports the growth of HEP-2 monkey kidney cells and primary explants of embryonic and adult human tissue. L-15 is buffered by phosphates and free base amino acids instead of sodium bicarbonate. This medium is designed for supporting cell growth in environments without CO2 equilibration.
Leibovitz's L-15 Mediumcontains no proteins, lipids, or growth factors. Therefore,Leibovitz's L-15 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS).
All raw material components used for Servicebio cell culture media products are screened through using strict quality control testing. Servicebio cell culture media are manufactured at a cGMP-compliant facility using water for injection, filtered through final0.1 μm sterile filtration and aseptic filled in a Hundred-stage FFU. The filter integrity tests are performed before and after filtration. Each lot of cell culture media are subjected to sterility test, pH, osmolality, endotoxin and cell culture test.
The major character of thisLeibovitz's L-15 Medium is as follows:
l With5.0 mM Galactose,2.05 mM L-glutamine,5.0 mM Sodium Pyruvate, withphenol red.
l Without Glucose, Sodium Bicarbonate andHEPES.
The complete formulation is available.
Storage
Storage conditions: 2℃ to 8℃, protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
For Research Use Only!
G4570 Leibovitz's L-15 Formula
|
mg/L |
mM |
mg/L |
mM |
||
|
Amino Acids |
Other Components |
||||
|
Glycine |
200.2 |
2.6667 |
D(+)Galactose |
900 |
5.00 |
|
L-Alanine |
225.0 |
2.5281 |
Phenol Red |
9.41 |
0.02657 |
|
L-Arginine HCl |
605.3 |
2.8736 |
Sodium Pyruvate |
550 |
5.00 |
|
L-Asparagine H2O |
284.3 |
1.8939 |
|||
|
L-Cysteine |
174.2 |
0.9917 |
|||
|
L-Glutamine |
300.3 |
2.0548 |
|||
|
L-Histidine |
250.0 |
1.6129 |
|||
|
L-Isoleucine |
250.3 |
1.9084 |
|||
|
L-Leucine |
125.2 |
0.9542 |
|||
|
L-Lysine HCl |
94.0 |
0.5137 |
|||
|
L-Methionine |
75.1 |
0.5034 |
|||
|
L-Phenylalanine |
125.1 |
0.7576 |
|||
|
L-Serine |
200.2 |
1.9048 |
|||
|
L-Threonine |
300.3 |
2.5210 |
|||
|
L-Tryptophan |
20.0 |
0.09804 |
|||
|
L-Tyrosine Disodium Salt |
372.3 |
1.6548 |
|||
|
L-Valine |
100.0 |
0.8547 |
|||
|
Vitamins |
|||||
|
Choline chloride |
1.00 |
0.007143 |
|||
|
D-Calcium pantothenate |
1.00 |
0.002096 |
|||
|
Folic Acid |
1.00 |
0.002268 |
|||
|
Niacinamide |
1.00 |
0.008197 |
|||
|
Pyridoxine hydrochloride |
1.00 |
0.004854 |
|||
|
Riboflavin 5'-phosphate Na |
0.10 |
0.000209 |
|||
|
Thiamine monophosphate |
0.94 |
0.002262 |
|||
|
i-Inositol |
2.00 |
0.011111 |
|||
|
Inorganic Salts |
|||||
|
Magnesium Sulfate (MgSO4) (anhyd.) |
97.7 |
0.8139 |
|||
|
Potassium Chloride (KCl) |
400.0 |
5.3333 |
|||
|
Sodium Chloride (NaCl) |
8000.0 |
137.93 |
|||
|
Sodium Phosphate dibasic (Na2HPO4) anhydrous |
189.9 |
1.3380 |
|||
|
Calcium Chloride (CaCl2) (anhyd.) |
140.0 |
1.2613 |
|||
|
Potassium Phosphate monobasic (KH2PO4) |
60.0 |
0.4412 |
|||
|
Magnesium Chloride (anhydrous) |
93.7 |
0.9863 |
|||
