Polybrene (10 Mg/mL)

Polybrene, also known as Hexadimethrine Bromide, is a class of polycationic polymers that are positively charged when dissolved and can bind to cell surface anions, and is commonly used in virus-mediated infections of mammalian cells by lentiviruses, adenoviruses and other viruses, and can significantly increase the efficiency of lentiviral and adenoviral infections of certain cells. The mechanism of action may be to promote adsorption by neutralizing the electrostatic repulsion between cell surface salivary acids and virus particles. Polybrene is also a well-known anti-heparin agent (heparin antagonist) and is commonly used to produce non-specifically agglutinated red blood cells. In addition, polybrene is also used in protein sequencing, because small doses of polybrene can significantly improve the degradation of peptides in automated sequencing analysis. The addition of polybrene to PVDF membranes also improves the affinity of the membrane. The concentration of the main component of this product is 10 mg/mL, and it is filtered by microporous membrane to remove bacteria.

Ship with wet ice; stored at 4℃, valid for 12 months; stored at -20℃, valid for 24 months. Avoid repeated freezing and thawing

1. According to the experimental requirements, Plant cells in the well plate and incubate plate overnight.

2. According to the MOI value obtained from relevant literature or pre-experiment, dilute the virus stock solution with complete medium to the desired concentration. And then add appropriate amount of Polybrene (10 mg/mL) to a final concentration of 2-12µg/ mL. (5-8 μg/mL is generally used).

3. Discard cell culture medium in the well plate. The virus solution-medium-polybrene mixture was added to the well plates, shaken gently and incubated in an incubator;

4. Incubate for 6-16 h in a cell culture incubator. Discard the culture medium containing virus, replace it with fresh complete medium (at this time, appropriate antibiotics may be added for screening), and continue to incubate (the incubation time of virus depends on the specific situation, if the cell is in good condition, the incubation time can be appropriately extended; Otherwise, it needs to be replaced with normal medium in advance).

5. According to the purpose of the experiment, the cells are collected or detected after a certain period of incubation.

1. Different cells have different sensitivities to Polybrene, so it is recommended to do cytotoxicity test for the first experiment.

2. If stored at -20℃, it is recommended that the storage be properly packed to avoid freeze - thaw cycles.

3. For your safty and health, please wear safety glasses, gloves, or protective clothing.

For Research Use Only!