Lentivirus Concentration Solution (5×)

The Lentivirus Concentration Solution (5×) provides a fast and simple method for concentrating all lentiviral stocks. Concentration is achieved by mixing a lentiviral supernatant with this concentration reagent, followed by a short incubation step and centrifugation in a standard centrifuge. The recovery rate can reach more than 90% and the virus titer can be increased by 100 folds.

Ship with wet ice; store at 2-8℃ for 12 months.

1. Harvest the lentivirus-containing supernatants. Cells and debris were removed by filtration using sterile filters with 0.45 μm membranes (low protein uptake PES membranes are recommended). And the PES filter membrane should be pre-cooled with 3-5 mL PBS once before passing through the virus stock solution.)

2. The lentiviral supernatant and 5× lentiviral precipitation concentrate were mixed in an inverted ratio of 4:1 (4 parts of viral supernatant and 1 part of viral concentrate), and the mixture was incubated at 4°C for 2 h, during which time it was inverted up and down for 10 times at 30 min intervals for mixing, or left to be incubated at 4°C overnight (an appropriately prolonged incubation time can improve the virus recovery rate).

3. After incubation for a certain period of time, centrifuge at 4℃, 8000 g for 10~15 min.

4. Carefully remove the supernatant, resuspend the lentiviral particles with 1/10~1/100 of the original volume of DMEM or PBS (G4202 is recommended) (in some cases, the precipitate may not necessarily be visible to the naked eye), gently blow to homogeneity, after resuspension of the precipitate, you can determine the titer of the concentrated lentiviral samples and then dispense them according to the experimental use volume and place them at -80℃ for storage (try to minimize the number of times that the lentiviruses have been repeatedly frozen and thawed, so as not to reduce the titer of the virus).

1. Filters and syringes are not provided with this kit. Please prepare your own filters and syringe.

2. Although the ingredients in this kit have a certain protective effect against lentivirus, please try to avoid lentivirus repeated freeze-thaw cycles.

3. For your safty and health, please wear safety glasses, gloves, or protective clothing.

For Research Use Only!