Exosome Concentration Solution

Exosomes are nanoscale membranous vesicles of uniform size, with a particle size ranging from 30 to 150 nm, actively secreted by cells into the extracellular space. This vesicle has its own specific biomarker, and contains proteins, mRNA, miRNA, lipids and other substances, which can participate in the process of cell growth, proliferation, differentiation, migration, angiogenesis and tumor growth through inter-cell material and signal communication.

This product is a common concentrated solution of Exosomes, which can quickly obtain a large number of complete Exosomes particles from liquid biological samples such as cell supernatant and animal serum.

Transportation at room temperature; Store at room temperature with a validity period of 12 months.

1. Sample pretreatment:

a) Low content exosome biological samples (using "Cell Supernatant" as an example): Take the cell supernatant, centrifuge at 4℃, 3000×g for 10 minutes to remove cell debris; Centrifuge the supernatant again at 4℃ and 10000×g for 20 minutes.Then filter the supernatant using a 0.22 μm filter membrane and purify it for later use.

b) High content exosome biological samples (using "Animal Serum" as an example): Take animal serum, centrifuge at 4℃, 3000×g for 10 minutes fist; Then Centrifuge the supernatant at 4℃ and 10000×g for 20 minutes.Last filter the supernatant using a 0.22 μm filter membrane. After preliminary purification, dilute the animal serum in a ratio of 1:9 to 1×PBS volume for later use.

2. Exosome concentration:

a) Take the processed biological sample and mix it with an equal volume of exosome concentrate. After intense vortex oscillation for 15 s-60 s, let it stand for 2 hours at 4 ℃ in a refrigerator (Optional: Vortex oscillation every 30 minutes during the stationary period).

b) Place the mixed solution in a centrifuge, centrifuge at 4 ℃ and 10000×g for 60 minutes; Discard the supernatant.

c) Add pre-cooled PBS buffer in a ratio of 1 x PBS volume=100:1 according to the volume of exosome concentrate, and resuspend and centrifuge for precipitation (note: this is equivalent to 100×concentration, and different volumes of PBS can be added as needed for resuspension).

d) Transfer the heavy suspension to a 1.5mL centrifuge tube and place it in a centrifuge. Centrifuge at 4 ℃ and 12000 rpm for 5 minutes; Collect supernatant.

e) The supernatant is rich in exosomes and can be used for subsequent experiments. It can also be packaged and stored at -80 ℃ for future use.

1. This product can be stored stably at room temperature for a long time. Please shake and mix well before use.

2. This product can be used as a supplement to the cell supernatant concentration in the G4113 exosome separation chromatography column (cell supernatant).

3. During the experiment, please wear lab clothes and disposable gloves to avoid contamination and ensure safety.

For Research Use Only!