Swe Recombinant Trypsin (10×)-EDTA, No Phenol Red

SweRecombinantTrypsin is an animal origin-free recombinant enzyme, it cleaves peptide bonds on the C-terminal side of lysine and arginine but with greater specificity than native trypsin preparations due to the superior purity.SweRecombinantTrypsin(10×)-EDTA, phenol red, is a 10×Swe RecombinantTrypsin formulated in DPBS/1 mM EDTA withoutphenolred. It can be diluted to 1×using a balanced salt solution containing 1.1mMEDTA under sterile conditions, or directly used for dissociation of strongly adherent cells.

Features of this product: Gentle on cells, Room-temperature stable, Easy to use, Animal Origin Free (AOF).

Store atroom temperature or 4℃ protect from light..4℃ is better for preservation.

Shelf life: 12 months.

Shipping conditions: Ambient.

1. (Optional) SweRecombinantTrypsin(10×), diluted to 1× with balanced salt solution containing 1.1 mM EDTA under sterile conditions before use, and can also be used directly without dilution for strongly adherent cells.

2. Remove the cell culture medium and wash the cells 1-2 times with sterile PBS or D-PBS free of Ca²⁺ and Mg²⁺.

3. Add appropriate volume (e.g., 5 mL in a 75 cm² flask) of SweRecombinantTrypsin to cover the cells. Incubate atroom temperature or 37°C until cells have detached. Observe cell monolayer using an inverted microscope to ensure complete cell detachment from the surface of the flask.

4. Add 5 mL of pre-warmed complete medium toflask. Collect the cell suspension in a centrifuge tube.

5. Centrifuge it at 800-1200 rpm/min for5-10 min,then discard supernatant and resuspend cell pellet with 2–5 mL of pre-warmed complete medium for passaging culture or subsequent experiments.

1. This product is very stable at room temperature, but it is still recommended to store it at 4°C. Avoid room temperature above 30℃. Avoid repeated freezing and thawing, if you need to store at -20℃.

2. This product is sterile and qualified by mycoplasma, endotoxin and other tests. Please pay attention to aseptic operation during use.

3. For your health and safety, please wear a lab coat and disposable gloves during operation..

For Research Use Only!