Prodoct Information
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Description |
CD45 rabbit polyclonal antibody |
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Protein full name |
Receptor-type tyrosine-protein phosphatase C |
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Synonyms |
PTPRC, B220, CD45, CD45R, GP180, L-CA, LCA, LY5, T200, protein tyrosine phosphatase, receptor type C |
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Immunogen |
KLH conjugated Synthetic peptide corresponding to Mouse CD45 |
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Isotype |
IgG |
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Purity |
Affinity purification |
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Subcellular location |
Membrane, Membrane raft |
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Uniprot ID |
P06800 |
Applications
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IHC/IF |
Mouse |
1: 1000-1: 4000 |
liver, lung, lymph node+LPS |
Background
Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity.
Images
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IHC analysis of CD45 (GB11066). Sample: Mouse liver (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 4000, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
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IHC analysis of CD45 (GB11066). Sample: Mouse lung (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 3000, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
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IHC analysis of CD45 (GB11066). Sample: Mouse spleen+lps (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 4000, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
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IF analysis of CD45 (GB11066). Sample: Mouse lymph node+lps (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 4000, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h. |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).
