Blocking Reagent

Blocking Reagent
Product Introduction:
Protein mixture, generally used as a blocking agent in Western Blot experiments (Note: Generally not used in ELISA and cannot be used for the detection of phosphorylated proteins or biotinylated proteins). Cat.No.:GC310001-100g
Brand:Servicebio
Spec.: 100 g
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Description
Technical Parameters

Product Introduction

 

Product Name

Cat. No.

Spec.

Blocking Reagent

GC310001-100g

100 g

GC310001-500g

500 g

 

Introduction

 

Blocking Reagent is generally used as a blocking solution in Western Blot experiments. The use of Blocking Reagent formulated as a solution as blocking agent or antibody dilution solution can effectively reduce the non-specific binding of primary or secondary antibodies and membranes, reduce the background and enhance the signal-to-noise ratio. TBST is commonly used as a solvent, and the recommended concentration is 3%-5%.

 

Basic Attributes

 

Name

Blocking Reagent

Synonym

Blocking Reagent

Purity

99%

Appearance (character)

White to light yellow powder

Storage conditions

Room temperature

Unit

Bottle

Spec.

100 g/500 g

Related categories

Biochemical reagents, other

Solubility

Soluble in water

Validity

12 months

 

Storage and Handling Conditions

 

Stored at room temperature; Valid for 12 months.

 

Handling Instruction


The usage and dosage of biochemical reagents are mainly determined based on the customer's experimental purposes and existing experimental methods in literature or books. The following applications are for reference only.

Generally used as a blocking solution in Western blot experiments. Dissolving Blocking Reagent in solution as a blocking agent or antibody dilution solution can effectively reduce nonspecific binding between the primary or secondary antibody and the membrane, decrease background noise, and enhance the signal-to-noise ratio. TBST is commonly used as the solvent, and a concentration of 3%-5% is recommended.

Can be used for preparing microbial culture media. In microbial culture, Blocking Reagent can be used alone or as one of the components in complex microbial culture media. For example, a 10% Blocking Reagentsolution prepared in test tubes can be used for the maintenance and amplification culture of lactobacilli, especially suitable for species differentiation within the Clostridium genus.

 

Note

 

Ready for use, prepared as needed, the solution cannot be stored for a long time.

For your safety and health, please wear a lab coat and disposable gloves.

 

Note: The product may be optimized and upgraded. The actual label information prevails.

 

For Research Use Only!

 

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