1× Citric Acid Antigen-Retrieval Solution (pH 6.0,Ready To Use)

After cells or tissues are fixed with paraformaldehyde, formalin or other aldehyde reagents, the formation of aldehyde bond and carboxymethyl bond of antigen determinant, as well as the cross-linking of protein quality control, cause the change of protein spatial structure, so that the antigen determinant is closed, and the binding point between antigen and antibody is reduced. Finally, the positive detection rate and staining intensity of antigen during immune labeling are relatively weak, showing false negative or low positive rate. The protein cross-linking caused by aldehydes is reversible. Under certain conditions, such as high temperature or protease hydrolysis, the original conformation of the protein can be restored. This process is antigen retrieval. Antigen repair is a key step in immunohistochemistry. There are many methods for antigen repair, and different methods can be selected according to the characteristics of the tissue type. Some are suitable for thermal repair, while others are suitable for enzymatic repair. The pH value of antigen repair solution also has many different choices due to the characteristics of antigen. The company provides a variety of antigen retrieval solutions with different pH values, which can meet the needs of a variety of antigen repair.

The main component of this product is 10 mM citric acid-sodium citrate, with a pH range of 5.9-6.1 @ 25℃, and it can be directly poured into the antigen retrieval container for slice retrieval.

Room temperature storage and transportation; Valid for 12 months.

1.Please select the appropriate antigen retrieval solution based on experimental requirements and antigen characteristics. If an acidic or alkaline retrieval solution is required, please refer to G1207, G1218, G1203, and G1206.
2.Please wear a lab coat and disposable gloves during operation.

For Research Use Only!