DsDNase (Thermolabile)

The Servicebio dsDNase (thermolabile) is a heat-sensitive double chain deoxyribonuclease, which is recombined and expressed by Pichia pastoris and derived from Shrimp. The double stranded deoxyribonuclease specifically cleaves the phosphate diester bond in double stranded DNA to produce oligonucleotides with 5'-phosphate and 3'-hydroxyl ends. This product has strong specificity, does not degrade RNA and single stranded DNA. It is inactivated at 55℃for 5 minutes. These features are very suitable for quick and safe removal of contaminating genomic DNA from RNA samples and simplify the workflow, and making genomic DNA remover and cDNA synthesis in a single tube.

Source: Shrimp derived deoxyribonuclease, recombinant expression of Pichia pastoris.

Definition of enzyme activity: One unit is the amount of enzyme required to increase the reaction system A260 by 0.001 per minute with double chain DNA as substrate at 25℃ and pH 5.0.

Purity: ≥ 95% by SDS-PAGE detection.

Inactivation or inhibition: A 5-minute incubation at 55°C for complete inactivation of DNase (thermolabile); High activity of metal ions, chelating agents, SDS and reducing agents can inhibit the enzyme activity of dsDNase (thermolabile).

Storage (Dillution) buffer: 25 mM Tris-HCl, 2 mM MgCl2, 10 mM NaCl, 0.01% Triton X-100, 50% glycerol, pH 7.5.

10×Reaction Buffer：200 mM Tris-HCl, 60 mM MgCl2, pH 8.3.

Ship with wet ice; Store at -20℃ valid for 12 months.

1. Add the following component into a sterile, nuclease-free tube on ice in the indicated order, mix gently and centrifuge briefly.

2. Incubate at 37℃ for 2 minutes.

3. If the treated RNA sample is ready for reverse transcription, heat at 55℃ for 5 minutes to fully inactivate dsDNase (thermolabile).

1. When using the product, the enzyme should be on ice and stored immediately after use at -20ºC.

2. For your safty and health,please wear safety glasses, gloves, or protective clothing.

For Research Use Only!