0.02% EDTA Cell Digestion Solution (Versene Solution)

0.02% EDTA Cell Digest (Versene Solution), also known as EDTA Cell Dissociation Solution, is composed primarily of 0.02% EDTA (~0.5 mM). It is prepared in calcium- and magnesium-free D-PBS, animal origin-free (AOF), phenol red-free, and Chemical defined (CD), a mild non-enzymatic ready-to-use cell digest. EDTA chelates bivalent metal ions such as Ca2+ and Mg2+ on the cell membrane, thereby breaking the intercellular connection and prompting the cell to dissociate into a single cell. This product has low toxicity, no protease activity, and will not destroy the membrane surface protein molecules. It is suitable for weak adherent cell lines (such as 293T, NIH/3T3, etc.), and is often mixed with trypsin products in a certain proportion.

Ship withwet ice; Store at 4℃ for 12 months.

Note: Note: The following protocols are primarily used for weaker adherent cell lines.

1. Remove the cell culture medium and wash the cells 1-2 times with sterile PBS or D-PBS free of Ca²⁺ and Mg²⁺.

2. Add 0.02%EDTA cell digestion solution (Versene solution) to cover the cells and leave at room temperature for 1-2 minutes, shaking the petri dish or culture bottle gently. Note: The digestion time of different cells is different. If the cell wall is firmly attached and difficult to digest, the treatment time or the amount of dissociation solution can be appropriately increased.

3. When the cells showed obvious shrinkage or morphological changes, aspirate the 0.02% EDTA cell digest, add appropriate amount of PBS or D-PBS, and blow the cells. Then centrifuge the cells at 800-1200 rpm/min for 3-5 min, resuspend the cells with appropriate amount of culture medium, and then use them for subsequent experiments.

4. If it is difficult to make the cells fall off the bottom of the plate when gently blowing with the pipette, indicating insufficient digestion time, add an appropriate amount of 0.02%EDTA cell digestive solution for re-digestion.

1. This product dissociates very gently and is not recommended for routine passaging of more strongly adherent cells.

2. Because EDTA is not neutralized, the digested cells need to be washed with PBS to remove EDTA, otherwise it will cause difficulty in cell adhesion.

3. This product is a sterile ready-to-use product and has been tested for mycoplasma, endotoxin, etc. Please pay attention to aseptic operation during use.

4. For your health and safety, please wear a lab coat and disposable gloves during operation.

For Research Use Only!